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KMID : 0881720180330020131
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Journal of Food Hygiene and Safety
2018 Volume.33 No. 2 p.131 ~ p.139
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Inhibition of Pancreatic Lipase Activity and Adipocyte Differentiation in 3T3-L1 Cells Treated with Purple Corn Husk and Cob Extracts
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Lee Ki-Yeon
Hong Soo-Young
Kim Tae-hee
Kim Jai-Eun
Park A-Reum
Noh Hee-Sun
Kim Si-Chang
Park Jong-yeol
Ahn Moon-Sub
Jeong Won-Jin
Kim Hee-Yeon
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Abstract
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Our review begins with the maize hybrid for grain, called ‘Seakso 1,’ which was developed in 2008 by the Gangwon Agricultural Research and Extension Services in Korea, and subsequently registered in 2011. In this study, we aimed to investigate the lipid metabolic enzyme activity and inhibitory effect on the adipocyte differentiation, in 3T3-L1 cells of the identified Seakso 1 corn husk and cob extracts (EHCS). We investigated the pancreatic lipase inhibitory effect and anti-adipogenic effect of EHCS.The lipid accumulation and adipocyte differentiation were measured by the procedure of Oil Red O staining, Real-time PCR and the Western blot analysis. The pancreatic lipase inhibitory activity of EHCS was measured at higher levels than those of the positive control (orlistat) at 100, 500, and 1,000 μg/mL. In particular, EHCS was noted as being significantly inhibited and including a measured adipocyte differentiation and lipid accumulation, when treated during the adipocyte differentiation process in 3T3-L1 cells. Based on the Oil Red O staining, EHCS inhibited lipid accumulation at 19.19%, 33.30% at 1000 μg/mL, 2000 μg/mL, respectively. The real-time PCR and Western blot analysis showed that EHCS significantly decreased in the mRNA expression and protein level of obesity-related factors, such as peroxisome-proliferatorsactivated-receptor- γ (PPARγ) and CCAAT enhancer-binding-proteins α (C/EBPα). This study potentially suggests that the Saekso 1 corn husk and cob extracts may improve lipid metabolism and reduce lipid accumulation.
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KEYWORD
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Purple corn, Anti-obesity, 3T3-L1, PPARγ, C/EBPα
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